Okay for example if you want a certain
book what would you do the first option
is put well library find the section
from which you want that book if it is a
sociology book we'll go to the social
algae level if it's a chemistry book
you'll put in chemistry
similarly in bitech or in biotechnology
if somebody wants a copy of a gene virus
bacterial DNA and genome they will do
biotech libraries biotech libraries are
also called as gene library gene library
is a place then you can get clones of
whatever you wished now gene libraries
are nothing but the collection of DNA
fragments from a particular species it
could be any species any particular DNA
chain gene libraries are of two types
one is genomic library and another is
 cDNA level there is a vast difference
between the two when I say genomic
library it means it is a collection of
clones which could be of virus bacteria
why no it's anything of that sort
now sitting in a lab when I say the word
cDNA cDNA means complementary DNA
serially library it consists the
collection of all the complementary DNA
not when I say DNA the complementary of
it is on the Indians double-stranded
whereas RNA is single-stranded if you
use an enzyme reverse transcriptase yes
the same enzyme which is available in
HIV or human immunodeficiency virus if
you use that same enzyme then you can
convert the single-stranded RNA to
double-stranded DNA so how this actually
cDNA library serving the process is very
very simple what we do is we have our
own single-stranded RNA we take that
single-stranded RNA we use the enzyme
reverse transcriptase the same enzyme
which HIV virus produces and uses in our
body to make copies of its own DNA
yes the same enzyme risk
muncipal for its on the enzyme which is
available at HIV virus so you take your
single standard item you use the enzyme
reverse transcriptase now this enzyme
reverse transcriptase can make
single-stranded DNA into the double
stranded Dean now once your
single-stranded DNA is made into double
stranded DNA what you do is you take out
the best idea and put it inside plasmids
now when I say plasmids plasmids were
nothing but Shock Trauma soman
self-replicating small biomolecules when
you put your double-stranded DNA inside
a plasmid and together your
double-stranded DNA as well as class
mate when you put into host organism
then the host will replicate your
plasmid will also replicate along with
that your double-stranded DNA will also
replicate so this is how or this is the
way plasmids
or the copy of double-stranded DNA are
made with the help of classmates as well
as host organs and this is how CDA
libraries ugly so let us see
let us see what was gene library it is a
collection of DNA fragments from a
particular species Nagi Larry could be
genomic library of cDNA library cDNA
library is collection of complementary
DNA
you
the steps of CDL like we are I'll fill
it and collect added which is
single-stranded RNA use reverse
transcriptase enzyme third step obtain
the double-stranded DNA fourth step
insert the DNA into plasma flip step
transfer it into host cell and the last
step you get off is multiple copies of a
double stranded game
the step of CD in the library making
heart actually the RNA of
single-stranded RNA in context to put
enzyme reverse transcriptase you obtain
on double-stranded DNA now this double
stranded DNA is inserted into a plasma
which is circular and small now this
double stranded DNA along with the
plasmid it's transferred into host cell
which would be equally obvious and then
at the end you get multiple copies thank
you
