Now let us study about Transgenic Plants
when is it Transgenic, Transgenic plants
are those plants or transgenic animals
are those animals which are made by
combination or you do a genetic
manipulation into transgenic plants a
live example or a classic example of
transgenic plants would be BT-Cotton
or bacillus thuringiensis con BT-
cotton is the cotton variety now readily
available initially the cotton that was
available was inside by insects was
eaten by insects so Biotechnologists
came up with something which is called
as BT-Cotton now how is BT- cotton made
and how insects can never ever attack
BT-Cotton, BT-cotton is made with the
help of two things one a plasmid which
is called as a TI plasmid which is
obtained from Agrobacterium and another is a
courage to which is often from basilisk
or in genesis what happens is that when
you put this regime into a plasmid you
insert this plasmid along with a gene
into the genome of the cell of the plant
cell multiple copies are made and pen
insect comes to buy bt cotton the
insects die of starvation the neck of
the insect is choked and the insect die
of starvation due to the presence of
crying so let us see how people cotton
is made transgenic plants an example of
this is BP cotton it is made by using TI
plasmid from agro bacterium and karachi
obtained from Maslin screen Genesis or
big so PT cotton is an amalgamation of
TI plasmid
plus Karachi
the steps are taken be a plasmid pick
desire gene which is courage now use
like is less restriction enzyme in the
process introduce the rDNA into host
cell and the last step now multiple
copies of the plasmid plus desire G will
be made
the steps of bt cotton I take P I
plasmid from agrobacterium cut it with
the restriction enzyme de agro bacterium
now this plasmid along with the scry
gene you have to integrate them to form
on DNA which is recombinant DNA
now this our DNA you have to put it into
plants and we thought that you know now
this sells the our DNA integrate into
the plant genome and make multiple
copies of itself and new PP cotton is
form of bacillus thuringiensis cotton is
for thank you
