Hello friends
Today we will see the confirmatory test of fermentation
By converting lime water into mily water
Fermentation is a process
In which microorganism obtained glucose from sample
To utilise for its own need
And convert it into ethanol and CO2
And some amount of energy also liberate
If we could confirm this CO2
Means if we prove that this gas is CO2
Then we can surely say that
The ongoing process is of fermentation process
for this we will use lime water
which will convert from colourless to milky white water
Transfer 50 ml of distilled water into a conical flask
Use only Distilled water as it is free from Microbs
Or you may boil and then cool the normal water and
Now transfer 05 gm glucose
Then after
Mix and shake the flask to dissolve it properly
Now transfer 01 gm of yeast powder
While transferring yeast into solution
Then yeast use the glucose and produce energy and CO2
To chek and confirm this
We are using a very simple method
A cork with perforation is taken
And connected with a transpirate pipe into it
And dipped into a beaker
Which is filled with lime water
Lime water is a solution of calcium hydroxide
You can take any quantity of calcium hydroxide
And dissolve in water
You can also use test tube for dipping
You will need less quantity of lime water in test tube
Now you can see that
We have fitted a perforated cork over funnel
So the CO2 which will release from this
That will move through lime water
and enter into lime water
And when this will enter in lime water
There will be bubbles generation
And this bubble will convert transparent lime water
into milky white water
You can see that there is one bubble
This bubble will move the process
and convert it into milky white
You can see the changes in colour
White colour will increase as the process increase
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
