Hi, I’m Kilian from Oculyze and now we’re going to show you a step-by-step guide on
how to use our device so you can get started with your measurements
Now, I’ll be showing you how to use the Oculyze Better Brewing 2.0
Let’s begin with dilution
This is what you’ll need to make sure your yeast is well mixed
Step 1: fill your measuring cylinder with 99 milliliters of water
Step 2: fill your pasteur pipette with yeast to 1 milliliter
Step 3: empty the pasteur pipette into the measuring cylinder
Step 4: run the solution in and out of the pipette three times to make sure it is completely empty
Step 5: take the pasteur pipette and stir a few times Now it’s diluted
Now it’s diluted
In order to determine the viability of the yeast, it must be stained
We recommend using methylene violet
Here is what you will need for staining
step 1: take 0.5 milliliters of the diluted yeast sample and put it into the reaction tube
Step 2: then take 0.5 milliliters of the methylene violet solution and put it into the same reaction tube
Step 3: Mix the sample by running the solution in and out of the pipette a few times
Now, let’s load the chamber
If you're only measuring concentration, use the diluted sample
If you're measuring concentration and viability, use the stained sample
Use the pasteur pipette to load a small sample Pipette this into either one of the chamber openings
capillary forces will pull the sample through the chamber
If you’re doing concentration, allow 1 minute for the sample to settle
If you’re doing concentration and viability, allow for a total of 5 minutes for the stain to take effect and for the sample to settle
Now that the chamber is ready let’s start measuring
Firstly, let’s plug our compatible device into the microscope
The microscope is powered by the mobile device and the red light will illuminate when the two are successfully connected
Step 1: open the app and check the settings
Here, you can change the language, how many images you capture, chamber height
your choice of staining dye, cell size slider, sharpness indicator, resolution
manual counting function and warning options
Step 2: On the main menu, you can choose to either
check your viability and concentration, check only your concentration, or review your history
Since we have stained our sample with methylene violet, we will be checking our concentration and viability
Step 3: Place the sample chamber under the microscope
Slide the sample chamber up too the first marking
lightly screwing it into place turning the focusing wheel counter clockwise
step 4: Continue focusing until cells come into focus
you will feel the wheel tighten slightly as the pad comes into contact with the chamber
Be sure not to over tighten, as this can damage the chamber or the optics
Step 5: Take the image. The sharpness indicator on the bottom left helps you to take optimally focused images
aim for the highest number possible This number will differ from image to image
Focused images should look like this
Click “keep” if you are happy with the image
Step 7: you must conduct this step five times
Turn the focusing wheel clockwise to release the chamber before moving it to the next marking
Click “keep” if you are happy with the image, each time moving the position of the chamber to the respective marking
Step 8: fill in the details of the analysis
In the sample field – enter the name of your sample 
Date and time are automatically entered
If you followed this video, you can enter 1 part sample and 99 parts water for the dilution
and 1 to 1 for the sample’s mix ratio of coloring agent
Include whatever you would like in the comment section
we recommend where the sample was taken from, the type of yeast and the generation of the yeast
Finally, press the “Next Step” button
Your results will then be sent to the cloud for analysis with the results being sent back to your device
This analysis and reporting will normally take less than a minute
The results of the analysis are easy to follow
On the top left of the screen in the green circle is the concentration of the sample in million cells per milliliter
In the tiny yellow circle is the percentage of these cells that are budding
In the violet circle is the viability percentage
When you scroll down you can view the breakdown of the cells by size with the histogram
showing a distribution of the cell sizes in the sample
The green bars indicate live cells, and the violet bars stacked on top show the distribution of dead cells
Scroll down further and you can see how the algorithm has identified the cells
The green cells are the living cells, the violet cells are dead, while the yellow colour indicates budding cells
The Pitch Rate Calculator is accessible through the gray arrow
The history enables the brewer to check on past results
Simply click on the analysis you want to check
To get the results of that analysis
in order to guarantee optimal analysis
The chamber must be cleaned and dried immediately after each use.
Step 1: Fill the syringe with distilled water
Step 2: Carefully rinse the chamber
Step 3: Use the bellow to dry the chamber
Carefully blow air through the chamber
and optionally use a small piece of tissue
or a cotton swab at the end to collect excessive water
