today I'm going to talk about CRISPR and
RNA interference in heart cells versus
RNA interference RNA interference is
used in studying the functions of genes
it works by shutting off gene expression
through the use of double-stranded RNAs
as you can see in the first image, Dicer
trims the double-stranded RNA to form
the micro RNA or si RNA these forms of
RNA are used in the RNA induced
silencing complex also known as risc
risc is seen in the second image in
yellow risc targets the mRNA to prevent
translation next is CRISPR scientists
will often insert guide DNA into the
cell and guide DNA recognizes specific
DNA sequences in the specific cells in
this case I chose the heart cells along
with it the cas9 is added the guide
RNA will locate the target DNA sequence
and the cas9 will cut or cleave the
sequence out then a new sequence can be
added in place of the old cut out
sequence to repair the enzyme in the
cell as you can see I was given an
example and I was asked to choose
between the two approaches CRISPR and
RNA interference and my favorite
eukaryotic cells and I went with CRISPR
and here's why to begin with CRISPR has
already been used to target HIV in
humans however RNA interference has had
some issues with some of the medicine
related trials for example with macular
degeneration it's been shown to cause
blindness and since heart cells are so
important to your body for many reasons
we don't want to risk anything going
wrong in addition RNA interference is
considered a knockdown gene expression
tool which means that it doesn't
completely stop gene expression
however CRISPR is considered a knockout
gene editing tool next RNA interference
has had some issues with delivery in
cases of tumors and cancer and it's hard
to get into a specific location which we
also don't want with the heart cells
because it's such an important part of
our body
and lastly RNA interference could also
be a problem if a gene would
accidentally similar seek
is also repressed and it could
cause off-target effects lastly to end
this off I included a couple of my
sources to reference for more
information thanks for watching
