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Engineering HSC and
IT-JEE Mains and advanced videos. Hello
students Gene transfer methods in
biotechnology we have did various
processes like production of bt cotton
or PCR now in all these methods there
was a way to transfer genes for example
in BT- cotton we have to transfer the
cryo gene into the Plantagenet in PCR
we have to transfer various nucleotide
into pasha now in biotechnology
variable you have to transfer certain
genes into Plantagenet there are
various methods follow the methods which
are followed our agro bacterium mediated
gene transfer then there is PGE mediated
gene transfer like Bozo mediated gene
transfer micro injections so on and so
on
Hello students women studying various
methods of gene transfer now if we talk
about BT- cotton in BT-Cotton we had
to transfer energy whenever transfer
crouching in bt cotton the most
important things for transfer our
Agrobacterium and back sister in genesis
if we have to transfer a hygiene into bt
cotton we need a plasmid that can
integrate into climb achievable
similarly in biotechnology there are
various methods by which you can
transfer gene into plant you know for
example PDG mediated transfer that is
poly ethylene glycol mediated transfer
now what polyethylene glycol mediator
transfer does is polyethylene glycol is
a chemical which is present inside the
cell children now when you take that
chemical externally and integrate your
DNA or genome into poly ethylene glycol
and then by the process of endocytosis
the DNA or the poly ethylene glycol will
get into it into plunge in the next
method is particle bombardment
when I say particle bombardment it means
taking a particle and forcing it on
pressurizing it with a lot of force onto
the plant cell in particle bombardment
the method or the technique that is used
is nothing but constant or you can say
gold or silver particles now gold and
silver particles are attached to DNA of
your genome and with high pressure they
are forced onto the plant cell or with
high density they are forced onto the
plant cell the next method is micro
injections Micro injections work
similarly like normal injections what we
do is we take a micropipette we take
certain amount of liquids which could be
5 ml and or something like that and we
introduce into the branch you know this
is the most safest and the easiest ways
next come liposome mediated gene
transfer
liposomes liposomes are nothing but they
are the part of the plant cell or animal
cells now when you take out liposome all
lipid packs or normal terms would call
it fatback's you pick a file back
lipid back attach your gene of interest
to that fired back or a lipid back and
then when you try to put it in into the
plancha you the plans you know will
easily take that the cause of the
phospholipid binder remember lipid and
liposomes they are connected to each
other so the liposome will move into the
plant cell last but not the least method
agrobacterium now Agrobacterium is a
soil bacteria which is found in the soil
this bacteria can cause tumor into the
plant now this bacteria has a plasma
which is call s TI plasmid you can
remove the TI plasmas from that to your
bacterium and introduce your gene of
interest into the ti plasmid and now the
CI plasmid along with the gene of
interest can be introduced into anytime
remember the Bt cotton or the basilisk
ER in genesis cotton was made this way
it has agrobacterium which give TI
plasmid bacillus thuringiensis which
regime and together it was made in
completely cotton of bachelors through
in genesis god so let us try and
understand what are the various gene
transfer methods
let us understand what are the various
string transfer methods the first one is
PG mediated update PG is nothing but
poly ethylene glycol polyethylene glycol
stimulates DNA uptake by endocytosis and
no means inside and cytosis means said
simple by lipid bilayer without damaging
the Dean the second method is particle
bombardment technique in this hold or
tungsten guns are coated with DNA and I
carried in the plant cell at a greater
speed
the third method is micro injections
this technique involves taking foreign
DNA using micro puppets the amount of
DNA transferred is 0.5 to 10 micro grams
of my goodness which is very simple the
fourth method is light pulse or mediated
gene transfer it involves enclosing DNA
in lipid packs and then transferring it
the last method is agro bacterium
mediated method in this agro bacterium
is the soil bacteria just found in this
soil which causes ground for tumor in
the root head from this bacterium a
plasmid is isolated which is TI plasmid
or transfer plasma this isolated class
made from it can be used to make dat
cotton of balusters in genesis cotton
thank you
