- [Instructor] So, let's start looking
at a short sequence of DNA,
and the letters I'm going to use,
these are the shorthands for
the various nucleotide bases
that make up a sequence of DNA.
So, let's say that I have some thymine,
thymine, cytosine,
guanine, cytosine, thymine,
adenine, thymine, thymine,
and let's throw another thymine in there.
So, that would be our sequence of DNA,
and what would be the
corresponding sequence
of RNA that it would be transcribed into?
If you remember this from previous videos,
pause this video and
try to figure that out.
Well, the key thing to appreciate is,
if we're talking base pairs in DNA,
adenine pairs with thymine,
cytosine pairs with guanine,
but if we're talking
about pairing into RNA,
well then, instead of thymine in the RNA,
you would have uracil.
So, the RNA here is,
well, the thymine in
the DNA would correspond
to an adenine in the RNA,
adenine, guanine,
cytosine, guanine, adenine,
and now, since this is an RNA strand,
instead of having a
thymine right over here,
this would be a uracil,
adenine, adenine, adenine.
So, this process that we just did,
this is transcription.
Transcription,
transcription
from DNA,
DNA to
RNA.
Now, the next step, if we're talking about
the whole process of, how
does this information actually
have an effect on the
body, is we're gonna go
from the RNA and translate that into
a protein.
And the way we do that, we've
seen this in previous videos,
is every three of these
bases, that's a codon,
and it codes for a particular amino acid.
Now, to figure out what
amino acid it codes for,
we look at an amino
acid translation table,
and there's different
types that you might see.
This is the most typical type,
so the first base is A, second base is A,
third base is G.
First base, A, second base, A,
we're in this cell, third base is G,
and so that will code for
the amino acid, lysine,
so we could write l-y-s,
short for lysine here.
And we could've also gotten that
from a different type
of translation table.
For example,
you might see a circular
one that looks like that,
but we would've gotten the same result.
AAG, start at the center, AAG
codes for lysine.
Then, the next codon,
and if you're getting
as excited about this as I am,
I encourage you to pause this video
and try to keep translating this.
The next codon is CGA.
CGA,
arginine.
Arginine, and then the next one is UAA.
UAA.
Well here, they have this
little black, circular dot,
what does that mean?
Well, that means stop codon,
and sometimes they'll just
write the word stop there.
So, this is stop.
There is not an amino acid called stop,
this actually signals
to, and this is happening
at a ribosome, this is signaling for
the translation process to stop,
this is the end of our amino acid chain,
of our polypeptide chain.
And so, we will stop right over there.
But now, let's do some interesting things.
Let's think about situations where
there are mutations,
where some of these bases,
maybe something gets inserted,
maybe something gets deleted,
maybe something gets swapped out.
And so, let's start with what's known
as a point mutation, so let's say this C
gets swapped out for an A.
Well, if that happened,
then on the RNA strand,
all of a sudden this would be a uracil,
and if that is a uracil, this
AAG would still be there,
coding for lysine, but this
second codon is now different.
What would it now code for?
Well, CUA.
CUA, it'll now code for
leucine instead of arginine.
Leucine,
l-e-u.
This is fairly typical for
a substitution mutation.
It might change a particular amino acid,
but sometimes, it could
be more significant.
For example,
if this G was swapped out for an A,
then this C on the RNA
would then be a U,
and then what would happen?
Well, this first codon
would still code for lysine,
but the second one would be UGA.
UGA.
Now, all of a sudden, it
codes for a stop codon,
and so, the actual translation
process would stop,
which could be a very, very big deal
if this DNA sequence,
if the normal, non-mutated polypeptide
had to keep going on, and on, and on.
Over here, it just happened
to have a stop codon next,
but you could imagine, if
they had just, you know,
another thousand codons before the end,
but all of a sudden, you had a
point mutation to stop early,
that would significantly
affect the protein
that it's coding for.
Now, another type of
mutation that typically
has a fairly significant affect
is a frameshift mutation,
and that's where something
gets inserted or deleted
and shifts everything.
So, for example, instead of the A
being swapped in for the G,
what if the A got inserted here?
So then, our sequence
would look like this.
T-T-C,
and then we have A, and
then you have G-C-T,
G-C-T-A-T-T-T.
So, what just happened here,
this was our original sequence,
but the A got inserted here,
it didn't replace the G, and so everything
got shifted to the right.
Now, what are we coding for?
Well, when we transcribe
to RNA, this will be
A-A-G-U-C-G-A-U-A-A-A,
and now this first codon
still codes for lysine,
we've seen that multiple times.
But, what about this second codon?
This second codon over here, UCG.
UCG,
that's serine,
we got a different amino acid.
And what's interesting is, it's not just
that one amino acid is changing,
we're gonna see that keeps happening.
So now, we have AUA.
AUA.
Here, we have isoleucine.
So, isoleucine,
right over here, which is different
than what we had before, we
don't have a stop codon anymore,
and we would keep going on and on.
And so, you could imagine
a frameshift mutation
where you either insert something
or you take it out so that
the whole frame gets shifted,
can have a dramatic impact on what
it will transcribe and then translate for.
Now, lucky for us, even though mutations
are always going on, there are
many proofreading mechanisms
in biological systems to
make them less frequent
than they otherwise would be,
and people are still understanding
how these proofreading
mechanisms fully happen.
Another thing to appreciate is,
we often associate a mutation
as
being equal to a bad thing,
and often times, it is a bad thing.
What used to be a functional protein
may no longer be a functional protein
because the amino acids,
the coding got stopped short
or there was a frameshift mutation
that's just coding for
completely different things.
So, sometimes it could be very bad,
and some diseases actually are caused
by strange mutations
like that, that show up.
Often times, the mutation
might not be a big deal.
Maybe something gets swapped out,
maybe only one amino acid changes
and it doesn't really change
the ability of the protein to do its job,
in which case it doesn't matter,
but every now and then,
a mutation can actually
be a good thing.
In fact, we need the mutation in order
to have variation in a population,
and variation is what natural selection
and evolution run off of.
If you don't have variation,
then you're not going
to have different things
that get selected in
different environments
and you're not going to have
that gradual change over time.
So, a big picture, hopefully
you got a better appreciation
for how transcription,
and then translation,
let me write that down.
And then, so that's
transcription from DNA to RNA,
and then this is translation.
Translation
from RNA
to protein,
to protein.
We have appreciation of how that happens,
we got appreciation of how to
use these translation tables,
but also how either a point mutation
or a frameshift mutation
can eventually affect
the protein that gets coded for.
