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hello students BT-Cottan our Bacillus Thuringiensis
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in Bacillus Thuringiensis this is the cotton
that is today available this cotton
cannot be eaten by any insects now how
is it possible initially that cotton
that was available would was eaten by
insects how is it possible that our
cotton which is now available
cannot be eaten or by any insects BT-
cotton is made with the help of a gene
called crize now what is the formality
on how does  BT-cotton is me to make
BT-cotton in organism is use which is
called as Bacillus Thuringiensis from
that organism Para genesis is isolated
now this cry gene is introduced into
Bacillus Thuringiensis or it is
introduced into the cotton plant now
this cotton plant will integrate the cry
gene into its gene then you cool the
cotton plant this line will glow along
with the Chi unit in it now when an
insect come and feeds on this cotton
black peg regime will get into the body
of the insect it will paralyze the god
of the insects and eventually the insect
will die because canal gene produces a
toxin which is called frog toxin inside
the gut of them organism and the gut of
the organism paralyzes and eventually
the organism that's so this is how bt
cotton works bt cotton is made using the
principles of biotechnology what we do
will be use in seei plasmid cryogen
ligases vectors and restriction enzymes
so let us study how beta cotton is made
Bakley cotton or nothing bacillus
thuringiensis
is a bacteria found in soil it produces
a protein or a gene which is falling
scratching and when an insect feed on bt
cotton the gut of the insects paralyzes
and the insect dies inside insects body
cream is converted in to production of
toxins
the process by which bt cotton or
bacillus thuringiensis cotton is made is
as follows we take agrobacterium a soil
bacteria from that p isolate TI plasmid
this TI plasmid is cut with a
restriction enzyme now what we do we
take the gene of interest or the Kuragin
now this cry gene is foot inside a plant
vector which is the TI plasmid and now
this guy gene along with the plant
vector is put into a plant cell and now
when the plant will grow the cry gene
initial which was used along with the
plant vector will integrate into plant
chili and this is how BP cotton is me
thank you
