Vector in Biotechnology we use vectors
for example in normal life when you have
to travel what you use use American
vehicle is a medium by which you
transfer or travel from one place to
another
similarly in biotechnology or in biology
if you have to carry one particular
molecule from one place to another place
you use a vetor or vevicals
now vectors are used to transfer cut
piece of DNA into from the into the host
organism now when the host organism
multiplies your gene of interest also
multiplies inside the host organism
along with the vectors now vectors
obviously there are Verity of vectors
available the most commonly used type of
vectors are 3 plasmids bacteriophage and
transposons
when I say plasmids plasmids are
obtained from Ecoline one second
plasmids are extra chromosome when I say
extra chromosomal it means they are
located outside the nucleus region they
are circular
they are self-replicating and they are a
part of Ecola example of plasmids would
be PUC PUC 19 or pbr3 22 now the second
type of vector which is widely available
is bacterial watch when I say
bacteriophage it means bacteria and fast
it is a bacteria that attacks a fudge
now bacteriophage is basically a virus a
type of a vector which is a virus that
is used against a bacteria it is a virus
that can multiply inside of bacteria
example where bacteriophages can be
widely used is HIV experiments or
bacterial files transformation
experiments now comes the third type of
vector the third type of vector was
transposon transposons are nothing but
jumping genes
transposons can jump from one place to
another place inside of DNA or
and integrate themselves whenever they
wish to combine use of transposon so
word in the experiment of God for
example by gamma Clinton did the
experiment and jumping genes was widely
used to bang on American corn was found
or the genetic variety from the blue
variety which was the initial Indian
variety of the phone
the yellow variety the genetically
modified corn or the American type of
converse form and your transposons they
made use in that so let us study what
vectors are
let us see what vector or vehicles are
but it is used to transfer the cut piece
of DNA on the side gene of interest into
host organisms basically vectors they
are carrier of vehicles which are used
to transfer things from one place to
another type of vectors which are
available a plasmid bacteriophage and
transposon you take a vector put your
gene of interest form our DNA and then
put it into host organism
let us see the various type of factors
available first one is plasmid they are
extra chromosome one self-replicating
small segments that are found in
organisms it could be a colon example is
fuck 90 the second one is bacteriophage
it is a wireless that multiplies inside
the body of bacteria example could be
HIV virus can be used as a bacteriophage
the third and the last one transposons
they are jumping genes that can
integrate in the genome transposons was
used in the cone expand thank you
