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Engineering HSC and IIT-JEE Mains and
Advanced videos. hello students today we
are studying chapter biochemistry of 	Cell
in that we are studying topic factors
affecting enzyme action what if I say
that there are certain things like
temperature pH ions and substrate
concentration that can stop enzyme
action or hamper enzyme action .
there are various parameters that affect
enzyme action or that slows the reaction
or working of an enzyme the most
important factor is temperature enzyme
action is greatly impacted or affected
by temperature at a temperature of 40 to
60 degree Celsius there is a loss of
enzyme action all the enzymes work
optimally at a temperature below 40 for
example the human body has a temperature
of 37 and in a human body
all the enzymes that work are injured
end of 37 to 39 the next measure is pH
at an optimum pH the activity of an
enzyme is maximum an enzyme could be
acidic basic or alkaline for example
Etzel is an acid so it will work better
into acidic environments where as NaOH
is a type of a base so it will work
better in basic environment most of the
enzyme works best between four to nine
pH and now enzyme function can be
greatly influenced by certain ion
concentration an important factor of
this could be H+ ions or hydrogen ion
concentration if hydrogen ion
concentration increases or decreases it
greatly hampers certain reactions inside
body this could be a mnemonic reactions
which are addition reactions or
catabolic reactions the last one would
be concentration of substrate if the
substrate concentration increases during
a particular reaction the speed of the
reaction would decrease more is the
substrate concentration whole will be
the time required to carry out the
reaction and if the substrate
concentration keeps on increasing beyond
a certain level there will be a point
where the enzyme will be fully saturated
when I say it will be fully saturated it
means it will be fully used up since
there is lot of substrate coming in for
a particular enzymes
so these are the factors that affect
enzyme functioning on dad stops enzyme
working at an optimum level so let us
see what are the factors that affect
enzyme function
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factors affecting enzyme action or
factors which hampered the working of an
enzyme these factors are temperature and
the action is greatly affected by
temperature at 40 to 60 degree Celsius
that is when the temperature increases
beyond 40 degree Celsius to 60 degree
Celsius then the loss of enzyme activity
the enzyme would denature it will not
function properly or the structure of an
enzyme would be hampered or destroy at
this temperature the next one is pH
optimum pH the activity of an enzyme is
maximum depending upon the type of the
enzyme whether it is acid a basic and or
alkali the enzyme will function
according to its pH at a pH of four
through nine enzymes function best
ion's enzyme activity is greatly
affected by h+ ions concentration of
hydrogen ion concentration concentration
of substrate if the substrate
concentration increases the rate of
enzyme reaction also Rises it means if
the substrate concentration increases
the reaction will be more slower since
there is less enzyme available or
required number of enzyme available for
a large number of substrate and there
will be a time when all the enzyme will
be used by a substrate which is
continuously increasing so for a
particular enzyme there should be a
particular substrate in particular
quantity and the quantity of enzyme and
substrate has to be parallel to speed of
the reaction
if the substrate concentration increases
the reaction will be soon so students in
this part we have studied the various
factors affecting enzyme I hope we all
are clear about this part of the chapter
thank you
