This video  goes through what we're looking for with a good streak isolation. So remember we've got
new culture added here at this dark vertical line.  And
after that we don't add any more new
culture or organism.  We simply sterilize our tool and spread it out
sterilize our tool, spread it out, sterilize our tool, spread it out.   When we get to this last
quadrant, we kind of fishtail and really try to spread it out.   So let's look at this on a plate
Here are some results here for streak isolation so you can see the vertical
or both of these. I'm going to angle that just a teeny bit more. And they can be slightly different and still come out quite well.
Then you've got your spreading it out spreading it out, spreading it out and
as you go into the very last area you can see they
fishtailed out on both of these.  And what you're looking for here is the ability to pick up
One colony
that's isolated and it's by itself we're isolating it.  So if i'm going let's say to this area
I really can't pick up on individual colony without touching others. Same here on this one.  But I just want to get
to one area where I've got what we call
isolated or spread out colonies and you see that on both these are different organisms so you can get different
colony sizes so the colony size is not significant.  It's just simply a matter of the
spacing between them that you can easily pick them up with the tool. And that's what a good streak isolation looks like.
