>> The main goal of
our laboratory is
to understand how the
B lymphocyte develops.
And to do that we have taken
an approach where we want
to study the processes
that are taking place
in the nucleus of the B cell.
These are cells from
the immune system
that produce antibody molecules,
these are the proteins
that cross-react with pathogens
when you get an infection.
In the nucleus, where most of
the action is happening in terms
of what the B cell will do,
we try to study what
those signals getting
from the outside are
changing in the nucleus
so that the cell can respond.
To understand how these
processes are orchestrated,
one thing that we've been
trying to develop is technique
to modify the genome at will.
And in order to do that we
have to develop a process
where we can introduce
specific mutations or deletions
across the genome at
a very specific site.
So one technique we have been
using is the TALEN(s) approach,
which are small scissors
that introduce DNA breaks
at those specific
sites so we can go in
and make modifications.
And what Kyongrim is doing
now is she's assembling those
scissors so we can do
this genome engineering.
Essentially, anything that
you do with a pipette,
in terms of moving liquids,
you can do with this machine.
For our purposes the main
function of this machine is
to assemble part of
the TALEN technology.
It's going to take -- pick up
samples from various locations,
pull them together,
and then allow us
to essentially do digestion and
ligation in the same reaction.
So for us, it's really
just built to move a lot
of different components one by
one by one, to assemble them.
And here is where the
data will be acquired
from those experiments.
And this is a deep sequencer.
It means that it can
sequence millions and millions
of small, short pieces of DNA.
And the data has to be
processed and analyzed.
So those are the next stages
in this whole experiment.
>> So this is a core switch.
This is where all the
elements of the system come in,
both storage systems
connect to this,
and each of the end
switches connect to this.
>> My name is Steve Fellini,
I'm the systems architect
of the Biowulf cluster
at the NIH.
It's used entirely for
scientific computing,
it's used by the intramural
researchers at NIH for just
about every sort of biomedical
application you can think of.
>> For this here, no,
because then this is going
to be totally washed out.
>> So this is where all
the data is being analyzed
with bioinformatic tools.
So this is the last stage before
we actually pull all these
discoveries together
into a complete story.
