
Chinese: 
[♪音乐声♪] [STEMCELL Technologies]
[技术指南] [分选细胞前去除人血液样本中的血小板]
血小板是血液循环中较小而富有粘性的细胞片段
可粘附着如单核细胞此等具有粘性的血细胞
[Vicki Stronge博士] [产品经理，免疫学]
由于血小板产生的细胞团块会影响某些细胞的分选
通常需在细胞分选前去除人血液样本中的血小板
本视频将演示如何在密度梯度离心后
使用低速旋转
减少新鲜人血样本的血小板污染
收集单个核细胞之前，通过移液管去掉大部分
富含血小板的黄色血浆层
请参照密度梯度离心液的产品信息说明
或我们的技术视频，了解该操作流程的更多信息
[将PBMC移入一个新试管]
用含2% FBS的PBS缓冲液冲洗收集的细胞

English: 
[♪ music plays throughout ♪] [STEMCELL Technologies]
[Technical Guide] [A Guide to Removing Platelets from Human Blood Samples Prior to Cell Separation]
Platelets are small, sticky cell fragments that circulate in blood
and can adhere to other sticky blood cells, such as monocytes.
[Vicki Stronge, PhD] [Product Manager, Immunology]
Because the resulting cell clumps can interfere with certain cell isolations,
it is often necessary to remove platelets from human blood samples prior to cell separation.
This video demonstrates how to use a slow spin
to reduce platelet contamination in fresh human blood samples
after density gradient centrifugation.
Pipette off and discard most of the yellow-colored platelet-enriched plasma layer
before isolating the mononuclear cells.
Please see your density gradient medium product information sheet
or our technical video for more details on this procedure.
[Transfer PBMC to a new tube]
Wash the collected cells in PBS containing 2% FBS.

English: 
Centrifuge the sample for 10 minutes at 120 x g with the brake off.
Carefully remove and discard the supernatant.
Repeat the wash process 2 more times.
Your purified sample is now ready for cell isolation and downstream applications.
We hope that you have found this video
on how to use a slow spin to reduce platelet contamination
in fresh human blood samples helpful.
For more technical tips, including videos covering other sample preparation procedures,
please visit our website at stemcell.com.
[STEMCELL Technologies] [Scientists Helping Scientists] [www.stemcell.com]

Chinese: 
关闭制动，120 xg离心10分钟
小心地移出并去掉上清液
再重复此冲洗过程两次
此时获得的纯化样本可立即用于细胞分选和其他下游应用
我们希望本视频演示的
如何使用低速旋转法减少新鲜人血样本的血小板污染
对您有所帮助
有关更多的技术提示，包括其他样本制备的视频等
请访问我们的网站www.stemcell.com
[STEMCELL Technologies] [Scientists Helping Scientists] [www.stemcell.com]
