Right so we're going to talk about a couple of ways in which you can grow yeast.
And, typically you would pick some yeast from a plate like this, in which we have colonies that have been streaked out and are growing up.
And you can either put these colonies, or some yeast from these colonies into this; a liquid media,
or you can restreak the colonies onto solid agar plates.
First of all lets try a streak from one plate onto a new plate
and to do this, I'm going to use one of these disposable loops.
Some of you might have the older wire loops, which you would use with a Bunsen burner
and would you sterilise the loop before making contact with the yeast,
but these disposable ones are great because you can just use them and throw them right away.
To keep the area sterile, I've got the Bunsen burner going again.
So I'm going to switch that back to the blue flame so it gives us the most amount of protection.
Gently lift off some yeast from the plate
and depending on what your experiment's going to be, you're going to streak your new plate a little bit differently.
But I'm going to just do a really simple streak, so open up the new dish
and gently streak the yeast in one dimension on the plate.
You can then rotate the dish a little bit
and streak again from the edge of your first streak,
rotate the dish again and do a third streak,
rotate the dish one more time
and streak, a little more loosely into the centre of the dish.
And that it! You've transferred yeast cells from one dish onto another solid surface.
This time we're going to take a colony and put it into some liquid media.
So I have some YPD media here that's been sterilised in the autoclave.
I'm going to decant some of the YPD media into the conical flask.
I'm going to use the Bunsen burner to just sterilise the neck of the bottle.
I'm then going to use a disposable tool to pluck a colony of yeast from our source dish,
give it a swirl around in the conical flask to get some of the yeast cells off.
And this flask is now ready to be labeled with my name, and the organism that we're growing up.
Right we've got our liquid culture, and I'm going to use this mountain of shaking incubators to grow up our cells.
And this will keep the cells aerated and in motion to give them the best environment in which they can grow.
The cells that we streaked onto this new YPD plate I'm going to place in this static incubator.
And I'll come back to those when they're ready to be used.
*Laughing off camera* "Don't do the walk off! It look's ridiculous, so cheesy!"
