Good afternoon. Today we're going to
learn about the basics of cell culturing.
To start, we're going to want to gather
all of your supplies so you're ready to
go within the biosafety cabinet. Next,
we're going to want to grab a pair of
gloves and spray them with 70% ethanol,
like so.  After you've sprayed and wiped down
your gloves you're going to want to move
to the biosafety cabinet. The biosafety
cabinet is this right here and you're
going to want to raise the sash until
you get to this mark right here the sash
height line you're going to raise it
until the lights of the cabinet turn on.
The cabinet will keep alarming until
you've reached the proper height and the
lights have turned off.  The cabinet
blower does not begin right away as
there will be about a 30 second delay.
Do NOT start moving into the cabinet until the blower has fully turned on.
After the blowers have turned on you're
going to want to spray the inside of the
cabinet with 70% ethanol to make sure
the area is sanitized properly
make sure to spray the entire area of
the cabinet including the sides of the
wall
you may leave the napkin inside the
biosafety cabinet just in case you
encounter any spills in the future.
After you have probably sanitized your
cabinet we will begin spraying all of
your supplies and putting them inside
the cabinet.
it is important to note whenever you are
working with your cells, that
you will never directly spray any of
your samples with the ethanol but every
fomite that you put inside there will
get sprayed with ethanol. Excluding the serological
pipettes, which you'll see in just a
moment.
we will want to grab serological pipette
tips: a 5mL, a 10 mL, and a 25 mL, and
you'll place these inside the Bio Safety Cabinet.
